Таурхолатовый бульон

Taurocholate Broth is useful for selective isolation of coliforms from water, milk and other food products. 

Состав**:

** Состав выверен и доведен до соответствия необходимым параметрам

Приготовление:

Suspend 40.0 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Принцип и оценка результата:

Taurocholate broth selectively supports growth of gram-negative coliforms from water, milk and other similar food products. The selective action of this medium is attributed to crystal violet and sodium taurocholate which inhibits most species of gram-positive bacteria. Lactose is a fermentable carbohydrate. Neutral red is the indicator dye in the media. Lactose fermenters acquire pinkish red colour due to production of acid. Sodium chloride maintains the osmotic balance at the medium.

Контроль качества:

Внешний вид порошка:

Pinkish yellow coloured, free flowing powder.

Цвет и прозрачность готовой среды:

Red coloured, clear solution without any precipitate.

Кислотность среды:

Reaction of 4.0% w/v aqueous solution is pH 7.4 ± 0.2 at 25°C.

Культуральные свойства:

Cultural characteristics observed after 18-48 hours at 35°C .

Условия и сроки хранения:

Порошок хранить при температуре ниже +30°С. Использовать до даты, указанной на этикетке. Готовую среду хранить при температуре +2…8°С.